Effectivness of giving clove oil as an anaesthetic for survival rate and number of leucocytes in cantang grouper (Epinephelus sp.) in the closed transportation

Cantang groupers is one of fish commodities that have high economic value. Live fish transport has several problems for fish and one of the problem is stress in fish. Many stressors can cause mortality of fish if the fish body can not tolerate it. One way that can be used to reduce stress on fish which can increase the value of survival and decrease leucocytess during the transport is giving clove oil as an anaesthetic. This study aim to determine the effect of clove oil as an anesthetic substance on the survival rate (SR) and number of leucocytes in cantang grouper ( Ephinephelus sp.) in the closed transport. The method is an experimental method with a completely randomized design (CRD), with five treatments and three replications. The main parameters observed were survival rate and leucocytes of cantang groupers. The data obtained will be analyzed using ANOVA followed by Duncan’s Multiple Range Test. The results showed that administration of clove oil as an anesthetic may improve the survival rate and lower the number of leucocytes of cantang grouper, after the closed transport for 10 hours. The highest survival rate is 86,67% and the Leucocytes is 2,35×10 4 cell/mm 3 with 10ppm of clove oil dosage.