Extraction and Estimation of Different Active Constituents present in the different brands of Green Tea by using Analytical Methods

Green tea (camellia sinensis. L) is one of the most popular highly used in nutritional supplement in the world. Ethanol and water were used as a solvent to extract active constituents from different brand of green tea like Lipton, Tetley, and Ecovalley natural green tea. The active constituents of green tea can be determined by analytical instruments like IR spectrophotometer used to know functional groups and TLC technique is used to know purity and no of different constituents of different brands of green tea. The absorbances can be determined by Colorimetry and UV spectroscopy, The DSC can be used to know the melting point of different constituents of different brands of green tea. The antioxidants present in the different brands of green tea estimated by using DPPH method. Predict the Immune booster activity of-(-) epigallo catechin gallate (EGCG) with target protein 3NG5 by docking study using the software swiss docking. By the above analytical methods, Ethanolic extract shows high percentage of yield than hot water extraction. Lipton green tea brand shows more active constituents compared to other brands of green tea.