New spectroscopic estimation of cefotaxime in pure and pharmaceutical formulation using environmental-friendly method

Simple and cost low, accurate and fast spectrophotometric methods were developed for cefotaxime determination. First method including change cefotaxime to a colored Fe (II) complex in the alkaline medium. Colored product with absorbance at λmax505 nm is reddish orange. Concentration ranged between (2-50 μg.mL-1), the Beer’s law obeyed with correlation coefficient 0.9995, molar absorptivity was 0.405×104 L.mol-1.cm-1, limit of detection 0.370μg mL-1 and limit of quantification as 1.123 μg mL-1. The second cloud point extraction method was used to estimate the trace quantity of the colored product in the first method, followed by UV-Vis spectrophotometer calculation. The calibration curve was the range of (2.5-30 μg.mL-1), the correlation coefficient and molar absorptivity were 0.9996 and 1.16×105L.mol-1.cm-1respictvely. The detection limit LOD and quantification limit LOQ were to be 0.026 and 0.079 μg.mL-1 respectively. This technique was effectively employed for cefotaxime detection within the pure and pharmaceutical preparations.