The effect of murashige and skoog (MS) modified medium and several types of auxins on the growth of stevia (Stevia rebaudiana bertoni) in vitro

The aims of this research is to testing the use of several types of auxins with the same concentration (0.2 ppm IAA; 0.2 ppm NAA; 0.2 ppm IBA) and modification of MS media (1/4 MS; 1 / 2MS; MS full ). Parameters observed included roots number, roots length, plantlets length and velocity of root emergence. The result shown that roots appeared influenced by the modification of MS media, the fastest root appearance was in the treatment using full MS and 1/2 MS on 14 and 15 DAS (day after showing). The several types of auxin and the modification of MS media were very significant. The number of roots parameter also resulted from the interaction between the auxin type and the modification of MS media which had a very significant effect. At the end of the observation (30 DAS) highest number of roots with an average of 4.23 was found in the using NAA treatment, with 1/2 MS. The modification of MS media is more optimal increasing roots length is at 1/2 MS. The modification of MS media which is more optimal in increasing length of plantlets at full media and was not significantly different with 1/2 MS.