Preparation of Mesoporous Organosilica-based Nanosystem for in vitro Synergistic Chemo- and Photothermal Therapy

Organic-inorganic hybrid mesoporous organosilica has gained more attention in biomedicine due to its high surface area, mesoporous channels, functional framework, and high drug loading capacity. In this study, disulfidebridged hybrid mesoporous organosilica nanoparticles（MONs） as nanocarriers were employed to construct a nanosystem(ICG/DOX-MONs@DNA20) for delivering drugs and photothermal agents, in which DNA molecules as "switches" were modified on the surface of MONs to control drug release. The results showed that the ICG/DOX-MONs@DNA20 nanosystem could increase the temperature to above 43 ℃ for photothermal therapy with near-infrared（NIR） laser irradiation. On the other hand, the ICG/DOX-MONs@DNA20 nanosystem exhibited a very slow release of DOX（12.3% in 6 h） at 37 ℃, but a rapid release of DOX（52.4% in 6 h） occurred at 43 ℃. Cell culture experiments indicated that the nanosystem can be internalized by HeLa cells, and exhibited an enhanced therapeutic efficacy of synergistic chemo-and photothermal therapy. Hence, the ICG/DOX-MONs@DNA20 nanosystem might be promising for synergistic chemo-and photo-thermal tumor therapy.