Characterization of Pollen Germination and Storage of Paeonia ludlowii

【Objective】Optimization of an in vitro germination protocol to effectively determine the viability of Paeonia ludlowii pollen,the effects of different storage conditions and incubation temperature on pollen longevity were compared to determine the appropriate temperature for pollen storage in the short,mid and long term,and the physiological mechanism of the programmed death of P. ludlowii pollen at different storage temperatures was analyzed,so as to provide a basis for cross breeding and germplasm resource conservation.【Method】The fresh pollen of P. ludlowii from Tibet was used to examine pollen morphology by scanning electronic microscope （SEM）. Pollen germination was characterized by in vitro culture to investigate the effects of different storage temperatures and times on pollen viability and activity of superoxide dismutase （SOD）,peroxidase （POD）,and catalase （CAT）,as well as the contents of ascorbic acid （AsA） and malondialdehyde （MDA）. 【Result】 The rate of pollen plumpness of P. ludlowii is high under