Annona muricata leaves ethanolic extract in water, n-hexane, and methanol fractions: Cytotoxicity assay, antioxidant activity, flavonoid content, polyphenol content, and acetogenin content

Cytotoxicity, antioxidant activity, and a total bioactive assay of the ethanolic extract of Annona muricata leave partitioned into water, n-hexane, and methanol fractions were investigated. The brine shrimp lethality test used for the cytotoxicity assay gave the LC50 values of 84 and 1 ppm for the n-hexane and methanol fractions, respectively. The antioxidant activity determined using DPPH reagent gave IC50 values of 40, 15, and 8 ppm for the n-hexane, water, and methanol fractions, respectively. In addition, the total polyphenol and the total acetogenin content of the methanol fraction were also the highest among the three fractions. These results indicate that the methanol fraction of the Annona muricata has the highest potential as a source for naturally occurring antitumor and antioxidant compounds.