0073 DYNAMIC CHANGES IN HUMAN INNATE AND ADAPTIVE IMMUNE CELL NUMBERS IN RESPONSE TO SLEEP EXTENSION AND SLEEP DEPRIVATION
Abstract Introduction: It has not been determined if innate and adaptive immune cell counts change following sleep extension (EXT). Here, we combine 2-days of baseline (8 h time-in-bed), 1-week of EXT (10 h time-in-bed) immediately followed by sleep deprivation (SD; 40 h) and recovery sleep (REC; 12...
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| Veröffentlicht in: | Sleep (New York, N.Y.) N.Y.), 2017-04, Vol.40 (suppl_1), p.A28-A28 |
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| creator | Simonelli, G Brager, A Stowie, A Prindle, N Duncan, EH Bergman, E Gad, M Powers, M Ephrem, D Payne, C Ratcliffe, RH Yarnell, AM Bergmann-Leitner, ES Capaldi, VF Balkin, TJ |
| description | Abstract
Introduction:
It has not been determined if innate and adaptive immune cell counts change following sleep extension (EXT). Here, we combine 2-days of baseline (8 h time-in-bed), 1-week of EXT (10 h time-in-bed) immediately followed by sleep deprivation (SD; 40 h) and recovery sleep (REC; 12 h time-in-bed). We characterized dynamic changes in human innate and adaptive immune cell numbers in response to bidirectional sleep homeostatic and circadian manipulations.
Methods:
A total of 8 participants (4 males, mean age 24.7) stayed in the sleep research facility for 11 nights. Plasma was drawn ~0730 upon awakening for baseline, EXT, and 24 h into SD; and ~1030 after recovery. Innate (monocytes and natural killer [NK] cells plus surface markers) and adaptive (B-cells and T-cells plus surface markers) cells were quantified by flow cytometry. Gating ensured exclusion of apoptotic cells.
Results:
Broadly, there was a change in the composition of the leukocyte populations across the study (p |
| doi_str_mv | 10.1093/sleepj/zsx050.072 |
| format | Article |
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Bergman, E ; Gad, M ; Powers, M ; Ephrem, D ; Payne, C ;
Ratcliffe, RH ; Yarnell, AM ; Bergmann-Leitner, ES ;
Capaldi, VF ; Balkin, TJ</creator><creatorcontrib>Simonelli, G ; Brager, A ; Stowie, A ; Prindle, N ; Duncan, EH ;
Bergman, E ; Gad, M ; Powers, M ; Ephrem, D ; Payne, C ;
Ratcliffe, RH ; Yarnell, AM ; Bergmann-Leitner, ES ;
Capaldi, VF ; Balkin, TJ</creatorcontrib><description>Abstract
Introduction:
It has not been determined if innate and adaptive immune cell counts change following sleep extension (EXT). Here, we combine 2-days of baseline (8 h time-in-bed), 1-week of EXT (10 h time-in-bed) immediately followed by sleep deprivation (SD; 40 h) and recovery sleep (REC; 12 h time-in-bed). We characterized dynamic changes in human innate and adaptive immune cell numbers in response to bidirectional sleep homeostatic and circadian manipulations.
Methods:
A total of 8 participants (4 males, mean age 24.7) stayed in the sleep research facility for 11 nights. Plasma was drawn ~0730 upon awakening for baseline, EXT, and 24 h into SD; and ~1030 after recovery. Innate (monocytes and natural killer [NK] cells plus surface markers) and adaptive (B-cells and T-cells plus surface markers) cells were quantified by flow cytometry. Gating ensured exclusion of apoptotic cells.
Results:
Broadly, there was a change in the composition of the leukocyte populations across the study (p<0.001; repeated measures ANOVA). Innate cells significantly decreased by ~25% from baseline levels across EXT. Monocytes decreased more quickly than NK cells relative to baseline levels (p<0.05, paired t-tests). Monocytes rapidly increased by ~25% from baseline levels 24 h into SD (p=0.027), despite a week of EXT. NK counts remained ~25% reduced from baseline levels 24 h into SD (p=0.043). Monocytes and NK cells returned to baseline levels during REC. Adaptive cells also decreased by about ~25% from baseline levels across EXT; B-cell counts modestly decreased midway through EXT (p=0.05, paired t-tests), whereas T-cell counts immediately declined and remained reduced from baseline levels across EXT (p<0.05). Adaptive cells returned to baseline levels during SD and subsequent REC.
Conclusion:
Sleep extension and deprivation had a significant effect on the number of innate and adaptive immune cells in the peripheral blood. Further analysis will look at changes in the functionality of these leukocyte populations.
Support (If Any):
Department of Defense Military Operational Medicine Research Program (MOMRP). NRC Research Associateship Program.</description><identifier>ISSN: 0161-8105</identifier><identifier>EISSN: 1550-9109</identifier><identifier>DOI: 10.1093/sleepj/zsx050.072</identifier><language>eng</language><publisher>US: Oxford University Press</publisher><subject>Sleep deprivation</subject><ispartof>Sleep (New York, N.Y.), 2017-04, Vol.40 (suppl_1), p.A28-A28</ispartof><rights>Sleep Research Society 2017. Published by Oxford University Press [on behalf of the Sleep Research Society]. All rights reserved. For permissions, please email: journals.permissions@oup.com 2017</rights><rights>Sleep Research Society 2017. Published by Oxford University Press [on behalf of the Sleep Research Society]. All rights reserved. For permissions, please email: journals.permissions@oup.com</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids></links><search><creatorcontrib>Simonelli, G</creatorcontrib><creatorcontrib>Brager, A</creatorcontrib><creatorcontrib>Stowie, A</creatorcontrib><creatorcontrib>Prindle, N</creatorcontrib><creatorcontrib>Duncan, EH</creatorcontrib><creatorcontrib>
Bergman, E</creatorcontrib><creatorcontrib>Gad, M</creatorcontrib><creatorcontrib>Powers, M</creatorcontrib><creatorcontrib>Ephrem, D</creatorcontrib><creatorcontrib>Payne, C</creatorcontrib><creatorcontrib>
Ratcliffe, RH</creatorcontrib><creatorcontrib>Yarnell, AM</creatorcontrib><creatorcontrib>Bergmann-Leitner, ES</creatorcontrib><creatorcontrib>
Capaldi, VF</creatorcontrib><creatorcontrib>Balkin, TJ</creatorcontrib><title>0073 DYNAMIC CHANGES IN HUMAN INNATE AND ADAPTIVE IMMUNE CELL NUMBERS IN RESPONSE TO SLEEP EXTENSION AND SLEEP DEPRIVATION</title><title>Sleep (New York, N.Y.)</title><description>Abstract
Introduction:
It has not been determined if innate and adaptive immune cell counts change following sleep extension (EXT). Here, we combine 2-days of baseline (8 h time-in-bed), 1-week of EXT (10 h time-in-bed) immediately followed by sleep deprivation (SD; 40 h) and recovery sleep (REC; 12 h time-in-bed). We characterized dynamic changes in human innate and adaptive immune cell numbers in response to bidirectional sleep homeostatic and circadian manipulations.
Methods:
A total of 8 participants (4 males, mean age 24.7) stayed in the sleep research facility for 11 nights. Plasma was drawn ~0730 upon awakening for baseline, EXT, and 24 h into SD; and ~1030 after recovery. Innate (monocytes and natural killer [NK] cells plus surface markers) and adaptive (B-cells and T-cells plus surface markers) cells were quantified by flow cytometry. Gating ensured exclusion of apoptotic cells.
Results:
Broadly, there was a change in the composition of the leukocyte populations across the study (p<0.001; repeated measures ANOVA). Innate cells significantly decreased by ~25% from baseline levels across EXT. Monocytes decreased more quickly than NK cells relative to baseline levels (p<0.05, paired t-tests). Monocytes rapidly increased by ~25% from baseline levels 24 h into SD (p=0.027), despite a week of EXT. NK counts remained ~25% reduced from baseline levels 24 h into SD (p=0.043). Monocytes and NK cells returned to baseline levels during REC. Adaptive cells also decreased by about ~25% from baseline levels across EXT; B-cell counts modestly decreased midway through EXT (p=0.05, paired t-tests), whereas T-cell counts immediately declined and remained reduced from baseline levels across EXT (p<0.05). Adaptive cells returned to baseline levels during SD and subsequent REC.
Conclusion:
Sleep extension and deprivation had a significant effect on the number of innate and adaptive immune cells in the peripheral blood. Further analysis will look at changes in the functionality of these leukocyte populations.
Support (If Any):
Department of Defense Military Operational Medicine Research Program (MOMRP). NRC Research Associateship Program.</description><subject>Sleep deprivation</subject><issn>0161-8105</issn><issn>1550-9109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkN9OgzAUxhujiXP6AN418Va2ltICl5XVjQQKGbDoVcO_Ji4qCC7RPb11-ABenXO-833nJD8AbjFaYOST5fjatv1-eRy_EEUL5NpnYIYpRZZv1udghjDDlocRvQRX47hHZnZ8MgNHhFwCV8-Sx2EAgw2Xa5HBUMJNEXNpGslzAblcQb7iaR7uBAzjuJACBiKKoCziB7E9BbYiSxOZCZgnMIuESKF4yoXMwkSe8pO2Euk23PHcqNfgQpevY3vzV-egeBR5sLGiZB0GPLJqTJFtEd1Qm-maeZXr1V7p1dqnTqVt7TNGtc0IbitUuS51apdVXoNtgmnZ0Jo1jDolmYO76W4_dB-HdvxU--4wvJuXyqaIOA7yHGpceHLVQzeOQ6tVP7y8lcO3wkj9IlYTYjUhVgaxydxPme7Q_8P-A-0MdWo</recordid><startdate>20170428</startdate><enddate>20170428</enddate><creator>Simonelli, G</creator><creator>Brager, A</creator><creator>Stowie, A</creator><creator>Prindle, N</creator><creator>Duncan, EH</creator><creator>
Bergman, E</creator><creator>Gad, M</creator><creator>Powers, M</creator><creator>Ephrem, D</creator><creator>Payne, C</creator><creator>
Ratcliffe, RH</creator><creator>Yarnell, AM</creator><creator>Bergmann-Leitner, ES</creator><creator>
Capaldi, VF</creator><creator>Balkin, TJ</creator><general>Oxford University Press</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope></search><sort><creationdate>20170428</creationdate><title>0073 DYNAMIC CHANGES IN HUMAN INNATE AND ADAPTIVE IMMUNE CELL NUMBERS IN RESPONSE TO SLEEP EXTENSION AND SLEEP DEPRIVATION</title><author>Simonelli, G ; Brager, A ; Stowie, A ; Prindle, N ; Duncan, EH ;
Bergman, E ; Gad, M ; Powers, M ; Ephrem, D ; Payne, C ;
Ratcliffe, RH ; Yarnell, AM ; Bergmann-Leitner, ES ;
Capaldi, VF ; Balkin, TJ</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1502-3fd526fc68b78c8a8cf954bf2f9665f2631eb0b7754c76b8d12315ad5c6d654a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Sleep deprivation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simonelli, G</creatorcontrib><creatorcontrib>Brager, A</creatorcontrib><creatorcontrib>Stowie, A</creatorcontrib><creatorcontrib>Prindle, N</creatorcontrib><creatorcontrib>Duncan, EH</creatorcontrib><creatorcontrib>
Bergman, E</creatorcontrib><creatorcontrib>Gad, M</creatorcontrib><creatorcontrib>Powers, M</creatorcontrib><creatorcontrib>Ephrem, D</creatorcontrib><creatorcontrib>Payne, C</creatorcontrib><creatorcontrib>
Ratcliffe, RH</creatorcontrib><creatorcontrib>Yarnell, AM</creatorcontrib><creatorcontrib>Bergmann-Leitner, ES</creatorcontrib><creatorcontrib>
Capaldi, VF</creatorcontrib><creatorcontrib>Balkin, TJ</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><jtitle>Sleep (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simonelli, G</au><au>Brager, A</au><au>Stowie, A</au><au>Prindle, N</au><au>Duncan, EH</au><au>
Bergman, E</au><au>Gad, M</au><au>Powers, M</au><au>Ephrem, D</au><au>Payne, C</au><au>
Ratcliffe, RH</au><au>Yarnell, AM</au><au>Bergmann-Leitner, ES</au><au>
Capaldi, VF</au><au>Balkin, TJ</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>0073 DYNAMIC CHANGES IN HUMAN INNATE AND ADAPTIVE IMMUNE CELL NUMBERS IN RESPONSE TO SLEEP EXTENSION AND SLEEP DEPRIVATION</atitle><jtitle>Sleep (New York, N.Y.)</jtitle><date>2017-04-28</date><risdate>2017</risdate><volume>40</volume><issue>suppl_1</issue><spage>A28</spage><epage>A28</epage><pages>A28-A28</pages><issn>0161-8105</issn><eissn>1550-9109</eissn><abstract>Abstract
Introduction:
It has not been determined if innate and adaptive immune cell counts change following sleep extension (EXT). Here, we combine 2-days of baseline (8 h time-in-bed), 1-week of EXT (10 h time-in-bed) immediately followed by sleep deprivation (SD; 40 h) and recovery sleep (REC; 12 h time-in-bed). We characterized dynamic changes in human innate and adaptive immune cell numbers in response to bidirectional sleep homeostatic and circadian manipulations.
Methods:
A total of 8 participants (4 males, mean age 24.7) stayed in the sleep research facility for 11 nights. Plasma was drawn ~0730 upon awakening for baseline, EXT, and 24 h into SD; and ~1030 after recovery. Innate (monocytes and natural killer [NK] cells plus surface markers) and adaptive (B-cells and T-cells plus surface markers) cells were quantified by flow cytometry. Gating ensured exclusion of apoptotic cells.
Results:
Broadly, there was a change in the composition of the leukocyte populations across the study (p<0.001; repeated measures ANOVA). Innate cells significantly decreased by ~25% from baseline levels across EXT. Monocytes decreased more quickly than NK cells relative to baseline levels (p<0.05, paired t-tests). Monocytes rapidly increased by ~25% from baseline levels 24 h into SD (p=0.027), despite a week of EXT. NK counts remained ~25% reduced from baseline levels 24 h into SD (p=0.043). Monocytes and NK cells returned to baseline levels during REC. Adaptive cells also decreased by about ~25% from baseline levels across EXT; B-cell counts modestly decreased midway through EXT (p=0.05, paired t-tests), whereas T-cell counts immediately declined and remained reduced from baseline levels across EXT (p<0.05). Adaptive cells returned to baseline levels during SD and subsequent REC.
Conclusion:
Sleep extension and deprivation had a significant effect on the number of innate and adaptive immune cells in the peripheral blood. Further analysis will look at changes in the functionality of these leukocyte populations.
Support (If Any):
Department of Defense Military Operational Medicine Research Program (MOMRP). NRC Research Associateship Program.</abstract><cop>US</cop><pub>Oxford University Press</pub><doi>10.1093/sleepj/zsx050.072</doi><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0161-8105 |
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| issn | 0161-8105 1550-9109 |
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| source | Oxford University Press Journals All Titles (1996-Current); Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Sleep deprivation |
| title | 0073 DYNAMIC CHANGES IN HUMAN INNATE AND ADAPTIVE IMMUNE CELL NUMBERS IN RESPONSE TO SLEEP EXTENSION AND SLEEP DEPRIVATION |
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