0073 DYNAMIC CHANGES IN HUMAN INNATE AND ADAPTIVE IMMUNE CELL NUMBERS IN RESPONSE TO SLEEP EXTENSION AND SLEEP DEPRIVATION

Abstract Introduction: It has not been determined if innate and adaptive immune cell counts change following sleep extension (EXT). Here, we combine 2-days of baseline (8 h time-in-bed), 1-week of EXT (10 h time-in-bed) immediately followed by sleep deprivation (SD; 40 h) and recovery sleep (REC; 12 h time-in-bed). We characterized dynamic changes in human innate and adaptive immune cell numbers in response to bidirectional sleep homeostatic and circadian manipulations. Methods: A total of 8 participants (4 males, mean age 24.7) stayed in the sleep research facility for 11 nights. Plasma was drawn ~0730 upon awakening for baseline, EXT, and 24 h into SD; and ~1030 after recovery. Innate (monocytes and natural killer [NK] cells plus surface markers) and adaptive (B-cells and T-cells plus surface markers) cells were quantified by flow cytometry. Gating ensured exclusion of apoptotic cells. Results: Broadly, there was a change in the composition of the leukocyte populations across the study (p