Chemical Profiling of Tylophora indica (Burm. F.) Merrill. Shoot Cultures Established Through Leaf Explant
Tylophora indica (Burm. F.) Merrill. is an important medicinal plant of Asclepiadaceae family. Leaf explants were utilized for establishment of shoot cultures in Murashige and Skoog’s medium fortified with sucrose (3%) and different cytokinins individually as well as in combinations. Optimum 28.90 ±...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences, India. Section B: Biological sciences India. Section B: Biological sciences, 2021-03, Vol.91 (1), p.37-43 |
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| container_title | Proceedings of the National Academy of Sciences, India. Section B: Biological sciences |
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| creator | Patel, Swati R. Pathak, Ashutosh R. Joshi, Aruna G. Shrivastava, Neeta Sharma, Sonal |
| description | Tylophora indica
(Burm. F.) Merrill. is an important medicinal plant of Asclepiadaceae family. Leaf explants were utilized for establishment of shoot cultures in Murashige and Skoog’s medium fortified with sucrose (3%) and different cytokinins individually as well as in combinations. Optimum 28.90 ± 0.41 shoots regenerated in medium supplemented with 6-benzyladenine (BA, 4 µM) and kinetin (Kn, 8 µM), when leaves of second node were placed adaxially with upright position for 8 weeks. Further transferring it to same medium for another 4 weeks formed 63.80 ± 1.45 shoots. The shoots were successfully rooted using earlier published media for the same plant and healthy rooted shoots were hardened and acclimatized to field. High performance thin layer chromatography fingerprint revealed that the in vitro shoots were able to synthesize metabolites at par with in vivo shoots. |
| doi_str_mv | 10.1007/s40011-020-01194-y |
| format | Article |
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(Burm. F.) Merrill. is an important medicinal plant of Asclepiadaceae family. Leaf explants were utilized for establishment of shoot cultures in Murashige and Skoog’s medium fortified with sucrose (3%) and different cytokinins individually as well as in combinations. Optimum 28.90 ± 0.41 shoots regenerated in medium supplemented with 6-benzyladenine (BA, 4 µM) and kinetin (Kn, 8 µM), when leaves of second node were placed adaxially with upright position for 8 weeks. Further transferring it to same medium for another 4 weeks formed 63.80 ± 1.45 shoots. The shoots were successfully rooted using earlier published media for the same plant and healthy rooted shoots were hardened and acclimatized to field. High performance thin layer chromatography fingerprint revealed that the in vitro shoots were able to synthesize metabolites at par with in vivo shoots.</description><subject>Behavioral Sciences</subject><subject>Benzyladenine</subject><subject>Biomedical and Life Sciences</subject><subject>Cytokinins</subject><subject>Explants</subject><subject>Kinetin</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Medicinal plants</subject><subject>Metabolites</subject><subject>Nucleic Acid Chemistry</subject><subject>Plant Biochemistry</subject><subject>Research Article</subject><subject>Shoots</subject><subject>Sucrose</subject><subject>Tylophora indica</subject><issn>0369-8211</issn><issn>2250-1746</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kE9LxDAQxYMouKz7BTwFvOihdZKmf3LUZVeFFQXXc8im6bZLtqlJC_bbG63gzTnMO8x7b-CH0CWBmADkt54BEBIBhSgoZ9F4gmaUphCRnGWnaAZJxqOCEnKOFt4fIEyWAmHFDB2WtT42Shr86mzVmKbdY1vh7WhsV1sncdOW4Yyv7wd3jPE6vsHP2rnGmBi_1db2eDmYfnDa45Xv5c40vtYl3tbODvsab7Ss8OqzM7LtL9BZJY3Xi1-do_f1art8jDYvD0_Lu02kaMLGSEmVSUV5DsAgy2glIaxCs5SoIisKWlScp7pikuU8oVyWZQI7uiOSJypRJJmjq6m3c_Zj0L4XBzu4NrwUlPE8zVMKRXDRyaWc9d7pSnSuOUo3CgLiG6uYsIqAVfxgFWMIJVPIB3O71-6v-p_UF1lQeco</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Patel, Swati R.</creator><creator>Pathak, Ashutosh R.</creator><creator>Joshi, Aruna G.</creator><creator>Shrivastava, Neeta</creator><creator>Sharma, Sonal</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20210301</creationdate><title>Chemical Profiling of Tylophora indica (Burm. F.) Merrill. Shoot Cultures Established Through Leaf Explant</title><author>Patel, Swati R. ; Pathak, Ashutosh R. ; Joshi, Aruna G. ; Shrivastava, Neeta ; Sharma, Sonal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c234y-cac6ac2970040662fa062f8e451c868828f995ef4a479329add30b2b1a93c3c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Behavioral Sciences</topic><topic>Benzyladenine</topic><topic>Biomedical and Life Sciences</topic><topic>Cytokinins</topic><topic>Explants</topic><topic>Kinetin</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Medicinal plants</topic><topic>Metabolites</topic><topic>Nucleic Acid Chemistry</topic><topic>Plant Biochemistry</topic><topic>Research Article</topic><topic>Shoots</topic><topic>Sucrose</topic><topic>Tylophora indica</topic><toplevel>online_resources</toplevel><creatorcontrib>Patel, Swati R.</creatorcontrib><creatorcontrib>Pathak, Ashutosh R.</creatorcontrib><creatorcontrib>Joshi, Aruna G.</creatorcontrib><creatorcontrib>Shrivastava, Neeta</creatorcontrib><creatorcontrib>Sharma, Sonal</creatorcontrib><collection>CrossRef</collection><jtitle>Proceedings of the National Academy of Sciences, India. Section B: Biological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Patel, Swati R.</au><au>Pathak, Ashutosh R.</au><au>Joshi, Aruna G.</au><au>Shrivastava, Neeta</au><au>Sharma, Sonal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chemical Profiling of Tylophora indica (Burm. F.) Merrill. Shoot Cultures Established Through Leaf Explant</atitle><jtitle>Proceedings of the National Academy of Sciences, India. Section B: Biological sciences</jtitle><stitle>Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci</stitle><date>2021-03-01</date><risdate>2021</risdate><volume>91</volume><issue>1</issue><spage>37</spage><epage>43</epage><pages>37-43</pages><issn>0369-8211</issn><eissn>2250-1746</eissn><abstract>Tylophora indica
(Burm. F.) Merrill. is an important medicinal plant of Asclepiadaceae family. Leaf explants were utilized for establishment of shoot cultures in Murashige and Skoog’s medium fortified with sucrose (3%) and different cytokinins individually as well as in combinations. Optimum 28.90 ± 0.41 shoots regenerated in medium supplemented with 6-benzyladenine (BA, 4 µM) and kinetin (Kn, 8 µM), when leaves of second node were placed adaxially with upright position for 8 weeks. Further transferring it to same medium for another 4 weeks formed 63.80 ± 1.45 shoots. The shoots were successfully rooted using earlier published media for the same plant and healthy rooted shoots were hardened and acclimatized to field. High performance thin layer chromatography fingerprint revealed that the in vitro shoots were able to synthesize metabolites at par with in vivo shoots.</abstract><cop>New Delhi</cop><pub>Springer India</pub><doi>10.1007/s40011-020-01194-y</doi><tpages>7</tpages></addata></record> |
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| subjects | Behavioral Sciences Benzyladenine Biomedical and Life Sciences Cytokinins Explants Kinetin Leaves Life Sciences Medicinal plants Metabolites Nucleic Acid Chemistry Plant Biochemistry Research Article Shoots Sucrose Tylophora indica |
| title | Chemical Profiling of Tylophora indica (Burm. F.) Merrill. Shoot Cultures Established Through Leaf Explant |
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