Chemical Profiling of Tylophora indica (Burm. F.) Merrill. Shoot Cultures Established Through Leaf Explant

Tylophora indica (Burm. F.) Merrill. is an important medicinal plant of Asclepiadaceae family. Leaf explants were utilized for establishment of shoot cultures in Murashige and Skoog’s medium fortified with sucrose (3%) and different cytokinins individually as well as in combinations. Optimum 28.90 ± 0.41 shoots regenerated in medium supplemented with 6-benzyladenine (BA, 4 µM) and kinetin (Kn, 8 µM), when leaves of second node were placed adaxially with upright position for 8 weeks. Further transferring it to same medium for another 4 weeks formed 63.80 ± 1.45 shoots. The shoots were successfully rooted using earlier published media for the same plant and healthy rooted shoots were hardened and acclimatized to field. High performance thin layer chromatography fingerprint revealed that the in vitro shoots were able to synthesize metabolites at par with in vivo shoots.