Transcriptional profiling of microglia in the injured brain reveals distinct molecular features underlying neurodegeneration

Neurotrauma has been recognized as a risk factor for neurodegenerative diseases, and sex difference of the incidence and outcome of neurodegenerative diseases has long been recognized. Past studies suggest that microglia could play a versatile role in both health and disease. So far, the microglial mechanisms underlying neurodegeneration and potentially lead to sex‐specific therapies are still very open. Here we applied whole transcriptome analysis of microglia acutely isolated at different timepoints after a cortical stab wound injury to gain insight into genes that might be dysregulated and transcriptionally different between males and females after cortical injury. We found that microglia displayed distinct temporal and sexual molecular signatures of transcriptome after cortical injury. Hypotheses and gene candidates that we presented in the present study could be worthy to be examined to explore the roles of microglia in neurotrauma and in sex‐biased neurodegenerative diseases. Main Points Microglia display distinct temporal and sexual molecular signatures of transcriptome after cortical injury. Hypotheses and gene candidates are presented to explore the roles of microglia in neurotrauma and in sex‐biased neurodegenerative diseases.