A molecular architectural design that promises potent antimicrobial activity against multidrug-resistant pathogens

Addressing the devastating threat of drug-resistant pathogens requires the discovery of new antibiotics with advanced action mechanisms and/or novel strategies for drug design. Herein, from a biophysical perspective, we design a class of synthetic antibacterial complexes with specialized architectur...

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Veröffentlicht in:NPG Asia materials 2021, Vol.13 (1), Article 18
Hauptverfasser: Yuan, Bing, Liu, Jiaojiao, Deng, Zhixiong, Wei, Lin, Li, Wenwen, Dou, Yujiang, Chen, Zhonglan, Zhang, Che, Xia, Yu, Wang, Jing, Zhang, Mengling, Yang, Kai, Ma, Yuqiang, Kang, Zhenhui
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Sprache:eng
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Zusammenfassung:Addressing the devastating threat of drug-resistant pathogens requires the discovery of new antibiotics with advanced action mechanisms and/or novel strategies for drug design. Herein, from a biophysical perspective, we design a class of synthetic antibacterial complexes with specialized architectures based on melittin (Mel), a natural antimicrobial peptide, and poly(ethylene glycol) (PEG), a clinically available agent, as building blocks that show potent and architecture-modulated antibacterial activity. Among the complexes, the flexibly linear complex consisting of one Mel terminally connected with a long-chained PEG (e.g., PEG 12k –1*Mel) shows the most pronounced improvement in performance compared with pristine Mel, with up to 500% improvement in antimicrobial efficiency, excellent in vitro activity against multidrug-resistant pathogens (over a range of minimal inhibitory concentrations of 2–32 µg mL −1 ), a 68% decrease in in vitro cytotoxicity, and a 57% decrease in in vivo acute toxicity. A lipid-specific mode of action in membrane recognition and an accelerated “channel” effect in perforating the bacterial membrane of the complex are described. Our results introduce a new way to design highly efficient and low-toxicity antimicrobial drugs based on architectural modulations with clinically available agents. Integration and design of existing function units into specialized architectures might show combined and even improved performances of the original components. Here we describe a serial of synthetic antibacterial complexes composed of melittin, a natural antimicrobial peptide, and poly(ethylene glycol), a clinical available agent, as building blocks, which show potent and architecture-modulated antibacterial activity against multidrug-resistant pathogens and decreased cytotoxicity.
ISSN:1884-4049
1884-4057
DOI:10.1038/s41427-021-00287-y