Collect the basic microbial information to evaluate the deep groundwater environment

Basic data on geological environment are collected and organized in relation to the geological disposal of the high-level radioactive waste. While the importance of the collecting microbial information has recognized in the recent international trends, microbiological knowledge for deep subsurface environments is currently limited. In order to make a database of basic microbial information, it is essential to systematize a series of analytical methods. In this paper, we reported the results of microbial analyses of deep subsurface groundwater. Groundwater sample was collected from deep well located in the coastal area of Horonobe, Hokkaido, Japan. Then, we proposed an analysis flow to obtain the density of microbes, community structure, activity, and community constituents in the subsurface environment as basic data on microbes in a single sampling opportunity.