Human Papillomavirus DNA Detection by Droplet Digital PCR in Formalin-Fixed Paraffin-Embedded Tumor Tissue from Oropharyngeal Squamous Cell Carcinoma Patients

Introduction High-risk human papillomavirus infection impacts staging and prognosis of oropharyngeal squamous cell carcinomas (OPSCCs). Determination of HPV status in tumor tissue by p16-immunohistochemistry (p16-IHC) can be challenging; therefore, complementary methodologies could be useful in a cl...

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Veröffentlicht in:Molecular diagnosis & therapy 2021, Vol.25 (1), p.59-70
Hauptverfasser: Schiavetto, Camila Marques, de Abreu, Priscila Marinho, von Zeidler, Sandra Ventorin, de Jesus, Laís Machado, Carvalho, Raiany Santos, Cirino, Maria Thereza, Carloni, Adriana Cruvinel, Oliveira, Cristina, Scapulatempo-Neto, Cristovam, de Almeida, Gisele Caravina, de Menezes, Nei Soares, Carvalho, André Lopes, Reis, Rui Manuel, de Carvalho, Ana Carolina
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Sprache:eng
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Zusammenfassung:Introduction High-risk human papillomavirus infection impacts staging and prognosis of oropharyngeal squamous cell carcinomas (OPSCCs). Determination of HPV status in tumor tissue by p16-immunohistochemistry (p16-IHC) can be challenging; therefore, complementary methodologies could be useful in a clinical setting. Objective To test for accuracy and clinical relevance of HPV-DNA detection in formalin-fixed and paraffin-embedded (FFPE) tumor samples by droplet digital PCR (ddPCR). Materials and Methods Fifty OPSCCs were tested for p16-IHC status followed by HPV-16/18 DNA detection/quantification in FFPE-recovered DNA using ddPCR. Accuracy for HPV status determination and association with patient information were also evaluated. Results 32.0% (16/50) of the cases were p16-IHC positive (p16 +), 42.0% (21/50) had detectable levels of HPV-16 DNA, and none were positive for HPV-18 DNA. A higher median viral load of HPV-16 DNA was observed in p16 + cases ( p
ISSN:1177-1062
1179-2000
DOI:10.1007/s40291-020-00502-6