Development of Aflatoxin B1 Aptamer Sensor Based on Iron Porphyrin Organic Porous Material

In this paper, using molybdenum disulfide doped with gold nanoclusters (Au NCs@MoS 2 ) as the substrate, an aflatoxin aptamer sensor was constructed utilizing iron porphyrin organic porous material modified with streptavidin and loaded with gold nano-bipyramids (SA-Au NBPs@ porous aromatic framework (PAF)-40-Fe) as a label. The catalyzed reduction current of hydrogen peroxide (H 2 O 2 ) by Au NBPs@PAF-40-Fe was detected which is proportional to the concentration of aflatoxin B 1 . Thereby, quantitative detection of aflatoxin B 1 (AFB 1 ) is achieved. Under the optimal conditions, the linear range of the sensor is 0.05 ~ 85 ng/mL with detection limit of 0.017 ng/mL and the linear correlation coefficient ( R 2 ) of 0.9825. The average recovery is 99.28 %. This aflatoxin aptamer sensor has good selectivity and provides a highly sensitive method for the detection of aflatoxin.