Active Temperature Regulation and Teamed Boronate Affinity-Facilitated Microelectrode Module for Blood Glucose Detection in Physiological Environment

[Display omitted] •A novel microelectrode module for blood glucose detection was fabricated.•Blood glucose detection in physiological environment was realized via teamed boronate affinity and active temperature regulation.•Sensitive, selective, and reliable detection towards blood glucose was achieved.•High-performance application of actual blood glucose detection from patients was attained. Physiological environment analysis is highly important to accurate detection of biological samples. In this work, a teamed boronate affinity-based molecular imprinting microelectrode module (MEM) with active temperature regulation was prepared for blood glucose detection in physiological environment. A silicon-based microelectrode array was utilized as working electrode, and arc-shaped electrodes on a printed circuit board contributed to a compact tri-electrode system. This module integrates a thermistor and a semiconductor cooler/heater, enabling active temperature regulation. Blood glucose were analyzed at physiological temperature. Physiological temperature retained primitiveness and avoided changing physicochemical properties of blood glucose. Meanwhile, constant temperature provided a constant adsorption capacity of functional materials towards glucose. Functional electrode materials, aminoferrocene-modified multi-walled carbon nanotubes (MWCNTs-AFC) and teamed boronate affinity-based molecular imprinting polymers modified multi-walled carbon nanotubes (MWCNTs-TBA-MIPs), were filled in the measurement cell to form MWCNTs-TBA-MIPs/AFC MEM. MWCNTs-TBA-MIPs/AFC MEM detected glucose in physiological environment within 10 min and possessed a wide linear range (1 ∼ 180 μM) and a low LOD (0.61 μM). With MWCNTs-TBA-MIPs/AFC MEM, blood glucose was determined selectively, eliminating the interferences from other co-existing molecules. Moreover, MWCNTs-TBA-MIPs/AFC MEM was employed to accurately measure the blood glucose in human serum, revealing its potential for biochemical detection.