Ni and TiO2 nanoparticles cause adhesion and cytoskeletal changes in human osteoblasts

Titanium-based alloys have established a crucial role in implantology. As material deteriorates overtime, nanoparticles of TiO 2 and Ni are released. This study is focused on the impact of TiO 2 and Ni nanoparticles with size of 100 nm on cytoskeletal and adhesive changes in human physiological and osteoarthritic osteoblasts. The impact of nanoparticles with concentration of 1.5 ng/mL on actin and tubulin expression and gene expression of FAK and ICAM-1 was studied. The cell size and actin expression of physiological osteoblasts decreased in presence of Ni nanoparticles, while TiO 2 nanoparticles caused increase in cell size and actin expression. Both cell lines expressed more FAK as a response to TiO 2 nanoparticles. ICAM-1 gene was overexpressed in both cell lines as a reaction to both types of nanoparticles. The presented study shows a crucial role of Ni and TiO 2 nanoparticles in human osteoblast cytoskeletal and adhesive changes, especially connected with the osteoarthritic cells. Graphical abstract