Synthesis and intensive analysis of antibody labeled single core magnetic nanoparticles for targeted delivery to the cell membrane

This work is dedicated to the synthesis and characterization of antibody labeled magnetic nanoparticles as promising tool for biomedical applications. Single core magnetic nanoparticles were synthesized by thermal decomposition technique, followed by surface modification with ligand based on polyethylene glycol derivative. Fluorochrome-labeled species-specific antibodies were covalently conjugated to MNPs through a polyethylene glycol linker. The immunofluorescent analysis showed the successful binding of anti-α-tubulin or anti-β-catenin antibodies conjugated MNPs to α-tubulin and β-catenin in 4T1 cells of mouse breast cancer.