Optically and Structurally Stabilized Plasmo‐Bio Interlinking Networks

Visualization of dynamic interlinking networks which respond and adapt to the constantly changing environment would be highly beneficial in developing new composite materials and active/responsive materials. Here, optically and structurally stabilized plasmo‐bio interlinking networks (PBINs) free from photobleaching for high resolution, long term visualization are reported. Necessary for structural and optical stability, a new stability algorithm to comprehensively quantify stability and detect minute instability undetectable by traditional methods is introduced. Biocompatible plasmonic gold nanorods (Bio‐AuNRs) are synthesized for high resolution, long term imaging by utilizing bromide‐free alternatives to achieve CTA+ free. Systematic physical, chemical, and biological characterizations reveal the structural and optical stability of Bio‐AuNRs required for constructing PBIN. Lastly, with actin as a model of interlinking networks of the cytoskeleton, optically and structurally stable PBIN (100% CTA+ free, 97% crosslinking rate) in applications as active/responsive materials, are demonstrated. Interlinking networks, such as the cytoskeleton, dynamically respond and adapt to the constantly changing environment. Detailed visualization of the complex dynamics of interlinking networks would be highly beneficial in developing composite materials and active/responsive materials. Optically and structurally stabilized plasmo‐bio interlinking networks free from photobleaching for high resolution, long term visualization are presented.