Determination of Four Amide Fungicides in Grape Wine by Gas Chromatography Coupled with Tandem Mass Spectrometry

To simultaneously determine the presence of four amide fungicides (amisulbrom, boscalid, fluopicolide, and silthiofam) in samples of grape wine, a method involving gas chromatography coupled with tandem mass spectrometry (GC-MS/MS) was developed. Samples of the grape wine were diluted with water, after which they were purified and concentrated through solid-phase extraction, and analyzed using GC-MS/MS. Isotope-labeled internal standards were used to correct the matrix effects. The limit of detection (LOD) and limit of quantification (LOQ) of all four amide fungicides was 0.6 μg kg −1 and 2.0 μg kg −1 . The average recoveries were in the range of 76.5–93.4% with relative standard deviations (RSDs) below 5.4%, which were measured at three concentration levels. Structural analysis of the four amide fungicides was carried out for the first time based on the MS/MS spectrogram of the compounds.