Microscale Atmospheric Pressure Plasma Jet as a Source for Plasma‐Driven Biocatalysis

The use of a microscale atmospheric pressure plasma jet (μAPPJ) was investigated for its potential to supply hydrogen peroxide in biocatalysis. Compared to a previously employed dielectric barrier discharge (DBD), the μAPPJ offered significantly higher H2O2 production rates and better handling of larger reaction volumes. The performance of the μAPPJ was evaluated with recombinant unspecific peroxygenase from Agrocybe aegerita (rAaeUPO). Using plasma‐treated buffer, no side reactions with other plasma‐generated species were detected. For long‐term treatment, rAaeUPO was immobilized, transferred to a rotating bed reactor, and reactions performed using the μAPPJ. The enzyme had a turnover of 36,415 mol mol−1 and retained almost full activity even after prolonged plasma treatment. Overall, the μAPPJ presents a promising plasma source for plasma‐driven biocatalysis. Plasma jets and enzymes! A microscale atmospheric pressure plasma jet was employed to generate H2O2 for biocatalysis. Unspecific peroxygenase from Agrocybe aegerita was immobilized and used in a rotating bed reactor system. Conversion of ethylbenzene to (R)‐1‐Phol using plasma‐generated H2O2 was performed with high enantioselectivity and satisfactory TON.