Freeze dried multicomponent inclusion complexes of piperine with cyclodextrin and hydrophilic polymers: Physicochemical characterization and In vivo anti-inflammatory activity

[...]they can reduce the mobility of the CD molecules and increase the solubility of drug-CD complexes by altering the hydration properties of CD molecules. [...]date, no one has investigated the effect of incorporation of hydrophilic polymers on the PIP solubilizing efficiency of CDs and the anti-inflammatory effect of the resultant complex. The prepared complexes were evaluated for the saturation solubility and drug content, and characterized by differential scanning calorimetry (DSC), X-ray powder diffractometry (XRPD), Attenuated total reflectanceFourier transform infrared spectroscopy (ATR-FTIR), and scanning electron microscopy (SEM). The method described by Higuchi and Connors28 was used for the phase solubility studies in distilled water at room temperature (25 ± 2°C).PIP, in excess quantity (50 mg), was added to a series of 20 mL of aqueous solutions containing various concentrations of ßCD or HPßCD (0, 0.002, 0.004, 0.006, 0.008 and 0.01 M) in the presence or absence of auxiliary substance PVP (0.5% w/w) or POLO (0.5% w/v). A sample (5mg) was sealed in an aluminum pan and subjected to heating at a rate of 10°C /min from 30 to 400°C under nitrogen atmosphere Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR): Attenuated Total Reflectance (ATR), in association with Fourier transform infrared spectroscopy (FTIR) assists in direct analysis of the solid or liquid samples and does not require any complex sample preparation procedure.