Digital holography applied to simultaneously measure the shape and the radial deformation of a blood vessel (ex-vivo)

•Simultaneous shape and deformation measurements in blood vessels (ex-vivo) using off-axis holography.•Compact digital holography setup to measure 3D information with only one camera.•Dual wavelength holography applied to measure the shape of biological tissues. Radial deformation of blood vessels has been measured by combining shape and deformation measurements. The difficulty of measuring both magnitudes simultaneously is related to the different order of magnitude. The veins have diameters up to 20 mm and suffer micrometric deformations due to cardiac movement. Temporal comparison of two-wavelength multiplexed holograms has been used for calculations. The radius value was calculated from the information of the vein shape, obtained by means of double wavelength holography, while the deformation of the vein has been measured with traditional holographic interferometry. In this work, both techniques have been combined using only one recording system. The technique has been tested in a latex tube and in a sheep aorta (ex-vivo). The experiments in both cases have been designed to simulate real patient situations. Differences found between the model and real vessels are presented in the paper.