Antibiotics resistance of extended spectrum beta lactamases uropathogenic Escherichia coli in Peshawar-Pakistan

Urinary tract infection (UTI) is predominantly caused by Escherichia coli (E. coli). E. coli is the major extended-spectrum beta-lactamases (ESBL) producing uropathogenic bacteria, leading to serious complications and limiting therapeutic management. This study was undertaken to measure the proportion of ESBL producing and antibiotic susceptibility pattern of uropathogenic E. coli in Peshawar. During the study duration, a total of 214 isolates of uropathogenic E. coli with urine samples were collected from patients. The urine samples were inoculated on the recommended media for the growth of bacteria. The bacteria growth was identified based on clinical laboratory standard institute (CLSI) protocol. The antibiotic susceptibility testing (AST) was carried out for E. coli using various discs of antibiotics. The ESBL producing E. coli were identified by double discs diffusion method. Among the studied isolates, 46.3% were collected from male patients. The highest percentage of patients was identified in the age group of 21-30 years. The female patients were observed more resistant to antibiotics. Similarly, ESBL producing isolates were more resistant than non-ESBL producing isolates. The E. coli isolates were more resistant to few antibiotics including nalidixic acid, cefuroxime sodium, cephradine, and ofloxacin while least to imipenem, fosfomycin, and meropenem. Conclusively, MDR and ESBLs producing E. coli isolates were observed more challenging in the studied region. Therefore, the early identification of ESBL producing and MDR E. coli isolates may be helpful to prevent further resistance in patients and will be helpful for better management of UTIs.