Novel fabrication of morphology tailored nanostructures with Gelatin/Chitosan Co-polymeric bio-composited hydrogel system to accelerate bone fracture healing and hard tissue nursing care management

[Display omitted] •Novel fabrication of Gelatin/chitosan bio-composited hydrogel matrix was consisting of nano-TiO2 particles.•The nano-crystalline structure and TiO2 loaded hydrogen morphology were confirmed•Enhanced in vitro cell survival and cell spreading on the prepared TiO2 incorporated hydrogel.•This material could be an effective biomaterial for the nursing management of bone fracture healing. In this study, we are successfully fabricated on a hydrogel consisting of TiO2 nanoparticles loaded onto a gelatin/chitosan matrix to control the acceleration of bone fracture healing and improved the nursing care applications. Each specimen (chitosan, gelatin and titanium dioxide) were characterized and confirmed by using different techniques, Fourier transforms infrared spectroscopy, X-ray diffraction analysis, Scanning Electron Microscopy with Elemental dispersive X-ray analysis, Thermo-gravimetric and Differential thermal analysis. In addition, the cell cytotoxicity results verified that the TiO2/gelatin-chitosan hydrogel are nontoxic to osteoblasts. And cell fixation outcome after 5 days of incubation condition revels that the enhanced in vitro cell survival and cell spreading on the prepared TiO2 incorporated hydrogel with respect to gelatin/chitosan hydrogel. Furthermore, TiO2/gelatin-chitosan hydrogel nanostructures can modulate the bone fracture healing, indicating a potential application on nursing care.