Effect of Dietary Calcium on Adipogenesis Program and Its Role in Adipocyte Dysfunction in Male Wistar Rats
Dietary calcium influences obesity-related adipocyte dysfunction by modulating body weight. The molecular mechanism behind this weight management is under intense investigation. In the present study, the authors investigated the effect of dietary calcium on peroxisome proliferator-activated receptor...
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| Veröffentlicht in: | National Academy of Sciences, India. Proceedings. Section B. Biological Sciences India. Proceedings. Section B. Biological Sciences, 2020-09, Vol.90 (3), p.631-639 |
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| description | Dietary calcium influences obesity-related adipocyte dysfunction by modulating body weight. The molecular mechanism behind this weight management is under intense investigation. In the present study, the authors investigated the effect of dietary calcium on peroxisome proliferator-activated receptor-γ (PPAR-γ)-mediated adipogenesis program and its role in adipocyte dysfunction by measuring the inflammatory and oxidative markers within the adipose tissue of male albino rats. Healthy male rats were fed with a control diet based on AIN-93, and either low-calcium (0.25%) or high-calcium (1.0%) diet for 90 days. A significant inverse (
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| doi_str_mv | 10.1007/s40011-019-01135-4 |
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p
< 0.05) relationship exists between the calcium intake and anthropometric parameters like adipose tissue mass and body weight. Adipose tissue PPAR-γ activity along with its downstream enzyme activities like glyceraldehydes 3 phosphate dehydrogenase, lipoprotein lipase as well as the protein expression of fatty acid-binding protein 4 and calcium-sensing receptor were significantly increased (
p
< 0.05) in the low-calcium-fed rats which in turn promoted the adipogenesis program. At the same time, protein expression of inflammatory markers like tumor necrosis factor-α and interleukin-6 and oxidative stress markers like lipid peroxidation and protein carbonylation were significantly increased (
p
< 0.05) in low-calcium-fed rats. These deleterious effects were suppressed by the high-calcium diet by preventing the activation of the adipogenesis program. Thus, the present study showed the involvement of dietary calcium against the PPAR-γ-mediated adipogenesis program as well as its role in adipocyte dysfunction by regulating the inflammatory and oxidative stress.</description><identifier>ISSN: 0369-8211</identifier><identifier>EISSN: 2250-1746</identifier><identifier>DOI: 10.1007/s40011-019-01135-4</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Adipocytes ; Adipogenesis ; Adipose tissue ; Behavioral Sciences ; Biomedical and Life Sciences ; Body weight ; Calcium (dietary) ; Calcium-binding protein ; Diet ; Enzymatic activity ; Fatty acid-binding protein ; Inflammation ; Interleukin 6 ; Life Sciences ; Lipase ; Lipid peroxidation ; Lipoprotein lipase ; Nucleic Acid Chemistry ; Oxidative stress ; Peroxisome proliferator-activated receptors ; Plant Biochemistry ; Protein expression ; Proteins ; Research Article ; Rodents ; Tumor necrosis factor-α</subject><ispartof>National Academy of Sciences, India. Proceedings. Section B. Biological Sciences, 2020-09, Vol.90 (3), p.631-639</ispartof><rights>The National Academy of Sciences, India 2019</rights><rights>The National Academy of Sciences, India 2019.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1854-ff5ebe6f35233c76460d4fad1e5930aeba029b2c4d95cae6a34e238409dc5de83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s40011-019-01135-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s40011-019-01135-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,23930,23931,25140,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Das, Sandeep</creatorcontrib><creatorcontrib>Choudhuri, Dipayan</creatorcontrib><title>Effect of Dietary Calcium on Adipogenesis Program and Its Role in Adipocyte Dysfunction in Male Wistar Rats</title><title>National Academy of Sciences, India. Proceedings. Section B. Biological Sciences</title><addtitle>Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci</addtitle><description>Dietary calcium influences obesity-related adipocyte dysfunction by modulating body weight. The molecular mechanism behind this weight management is under intense investigation. In the present study, the authors investigated the effect of dietary calcium on peroxisome proliferator-activated receptor-γ (PPAR-γ)-mediated adipogenesis program and its role in adipocyte dysfunction by measuring the inflammatory and oxidative markers within the adipose tissue of male albino rats. Healthy male rats were fed with a control diet based on AIN-93, and either low-calcium (0.25%) or high-calcium (1.0%) diet for 90 days. A significant inverse (
p
< 0.05) relationship exists between the calcium intake and anthropometric parameters like adipose tissue mass and body weight. Adipose tissue PPAR-γ activity along with its downstream enzyme activities like glyceraldehydes 3 phosphate dehydrogenase, lipoprotein lipase as well as the protein expression of fatty acid-binding protein 4 and calcium-sensing receptor were significantly increased (
p
< 0.05) in the low-calcium-fed rats which in turn promoted the adipogenesis program. At the same time, protein expression of inflammatory markers like tumor necrosis factor-α and interleukin-6 and oxidative stress markers like lipid peroxidation and protein carbonylation were significantly increased (
p
< 0.05) in low-calcium-fed rats. These deleterious effects were suppressed by the high-calcium diet by preventing the activation of the adipogenesis program. Thus, the present study showed the involvement of dietary calcium against the PPAR-γ-mediated adipogenesis program as well as its role in adipocyte dysfunction by regulating the inflammatory and oxidative stress.</description><subject>Adipocytes</subject><subject>Adipogenesis</subject><subject>Adipose tissue</subject><subject>Behavioral Sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Body weight</subject><subject>Calcium (dietary)</subject><subject>Calcium-binding protein</subject><subject>Diet</subject><subject>Enzymatic activity</subject><subject>Fatty acid-binding protein</subject><subject>Inflammation</subject><subject>Interleukin 6</subject><subject>Life Sciences</subject><subject>Lipase</subject><subject>Lipid peroxidation</subject><subject>Lipoprotein lipase</subject><subject>Nucleic Acid Chemistry</subject><subject>Oxidative stress</subject><subject>Peroxisome proliferator-activated receptors</subject><subject>Plant Biochemistry</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Research Article</subject><subject>Rodents</subject><subject>Tumor necrosis factor-α</subject><issn>0369-8211</issn><issn>2250-1746</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LAzEURYMoWGr_gKuA69F8zseytFULFaUoLkMm81JS20lNZhb996ZOwZ0Pwlvk3PvgIHRLyT0lpHiIghBKM0Kr9CiXmbhAI8YkyWgh8ks0IjyvspJReo0mMW5JmlwSKsoR-lpYC6bD3uK5g06HI57pnXH9HvsWTxt38BtoIbqI34LfBL3Hum3wsot47XeA3Rkyxw7w_Bht35rOpWj6eNEJ-HQxteK17uINurJ6F2Fy3mP08bh4nz1nq9en5Wy6ygwtpcislVBDbrlknJsiFzlphNUNBVlxoqHWhFU1M6KppNGQay6A8VKQqjGygZKP0d3Qewj-u4fYqa3vQ5tOKiZ48sALfqLYQJngYwxg1SG4fRKgKFEnr2rwqpJX9etViRTiQygmuN1A-Kv-J_UDf5J6mw</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Das, Sandeep</creator><creator>Choudhuri, Dipayan</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20200901</creationdate><title>Effect of Dietary Calcium on Adipogenesis Program and Its Role in Adipocyte Dysfunction in Male Wistar Rats</title><author>Das, Sandeep ; Choudhuri, Dipayan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1854-ff5ebe6f35233c76460d4fad1e5930aeba029b2c4d95cae6a34e238409dc5de83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adipocytes</topic><topic>Adipogenesis</topic><topic>Adipose tissue</topic><topic>Behavioral Sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Body weight</topic><topic>Calcium (dietary)</topic><topic>Calcium-binding protein</topic><topic>Diet</topic><topic>Enzymatic activity</topic><topic>Fatty acid-binding protein</topic><topic>Inflammation</topic><topic>Interleukin 6</topic><topic>Life Sciences</topic><topic>Lipase</topic><topic>Lipid peroxidation</topic><topic>Lipoprotein lipase</topic><topic>Nucleic Acid Chemistry</topic><topic>Oxidative stress</topic><topic>Peroxisome proliferator-activated receptors</topic><topic>Plant Biochemistry</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Research Article</topic><topic>Rodents</topic><topic>Tumor necrosis factor-α</topic><toplevel>online_resources</toplevel><creatorcontrib>Das, Sandeep</creatorcontrib><creatorcontrib>Choudhuri, Dipayan</creatorcontrib><collection>CrossRef</collection><jtitle>National Academy of Sciences, India. Proceedings. Section B. Biological Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Das, Sandeep</au><au>Choudhuri, Dipayan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Dietary Calcium on Adipogenesis Program and Its Role in Adipocyte Dysfunction in Male Wistar Rats</atitle><jtitle>National Academy of Sciences, India. Proceedings. Section B. Biological Sciences</jtitle><stitle>Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci</stitle><date>2020-09-01</date><risdate>2020</risdate><volume>90</volume><issue>3</issue><spage>631</spage><epage>639</epage><pages>631-639</pages><issn>0369-8211</issn><eissn>2250-1746</eissn><abstract>Dietary calcium influences obesity-related adipocyte dysfunction by modulating body weight. The molecular mechanism behind this weight management is under intense investigation. In the present study, the authors investigated the effect of dietary calcium on peroxisome proliferator-activated receptor-γ (PPAR-γ)-mediated adipogenesis program and its role in adipocyte dysfunction by measuring the inflammatory and oxidative markers within the adipose tissue of male albino rats. Healthy male rats were fed with a control diet based on AIN-93, and either low-calcium (0.25%) or high-calcium (1.0%) diet for 90 days. A significant inverse (
p
< 0.05) relationship exists between the calcium intake and anthropometric parameters like adipose tissue mass and body weight. Adipose tissue PPAR-γ activity along with its downstream enzyme activities like glyceraldehydes 3 phosphate dehydrogenase, lipoprotein lipase as well as the protein expression of fatty acid-binding protein 4 and calcium-sensing receptor were significantly increased (
p
< 0.05) in the low-calcium-fed rats which in turn promoted the adipogenesis program. At the same time, protein expression of inflammatory markers like tumor necrosis factor-α and interleukin-6 and oxidative stress markers like lipid peroxidation and protein carbonylation were significantly increased (
p
< 0.05) in low-calcium-fed rats. These deleterious effects were suppressed by the high-calcium diet by preventing the activation of the adipogenesis program. Thus, the present study showed the involvement of dietary calcium against the PPAR-γ-mediated adipogenesis program as well as its role in adipocyte dysfunction by regulating the inflammatory and oxidative stress.</abstract><cop>New Delhi</cop><pub>Springer India</pub><doi>10.1007/s40011-019-01135-4</doi><tpages>9</tpages></addata></record> |
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| subjects | Adipocytes Adipogenesis Adipose tissue Behavioral Sciences Biomedical and Life Sciences Body weight Calcium (dietary) Calcium-binding protein Diet Enzymatic activity Fatty acid-binding protein Inflammation Interleukin 6 Life Sciences Lipase Lipid peroxidation Lipoprotein lipase Nucleic Acid Chemistry Oxidative stress Peroxisome proliferator-activated receptors Plant Biochemistry Protein expression Proteins Research Article Rodents Tumor necrosis factor-α |
| title | Effect of Dietary Calcium on Adipogenesis Program and Its Role in Adipocyte Dysfunction in Male Wistar Rats |
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