Steps towards standardized quantification of adult neurogenesis

New neurons are generated in adult mammals. Adult hippocampal neurogenesis is considered to play an important role in cognition and mental health. The number and properties of newly born neurons are regulatable by a broad range of physiological and pathological conditions. To begin to understand the underlying cellular mechanisms and functional relevance of adult neurogenesis, many studies rely on quantification of adult-born neurons. However, lack of standardized methods to quantify new neurons is impeding research reproducibility across laboratories. Here, we review the importance of stereology, and propose why and how it should be applied to the study of adult neurogenesis. Adult neurogenesis is involved in several physiological and pathological processes, however standardization for the quantification of new born neurons is lacking. Here, the authors provide guidance to improve reproducibility and rigour in cell quantification.