The complete mitochondrial genome of Juema pig (Suina:Suidae)

Juema pig ( Suina:Suidae ), a rare, miniature and primitive species, has been living and razing on the Qinghai-Tibet plateau completely wild for thousands of years. Owing to the cold plateau climate, low energy, and low carbohydrate diet, Juema pig has some unique genetic characteristics that differentiate it from other pig breeds. If the species should not be protected in a timely manner, it would become extinct in the future. In June 2006, No. 662 announcement of the People’s Republic ofChina has included the Tibetan pigs in the national protection list of poultry genetic resources . Developing reasonable conservation and utilization measures for Juema pig requires deeper understanding of molecular genetics. The complete mitochondrial genome of Juema pig was determined in this study. Similar to other vertebrate, the mitogenome is 16,690 bp in length and contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a D-loop region. The overall base composition of the H-strand is 34.76% for A, 26.17% for C, 13.24% for G and 25.83% for T. To ensure the sequence is reliable, phylogenetic tree was constructed using MEGA 6 with Neighbor-Joiningmethod (NJ) methods, demonstrating that Juema pig was clustered in subfamily Suina .