Immunohistochemical Analysis of the Metabolic Phenotype of Adrenal Cortical Carcinoma

Metabolic reprogramming is a cellular process contributing to carcinogenesis. However, it remains poorly understood in adrenal cortical carcinoma (ACC), an aggressive malignancy with overall poor prognosis and limited therapeutic options. We characterized the metabolic phenotype of ACC, by examining the immunoprofile of key proteins involved in glucose metabolism, hexokinase (HK1), pyruvate kinase (PKM1, PKM2), succinate dehydrogenase (SDHB), and phospho-S6 ribosomal protein (pS6), in a tissue microarray of 137 adrenal cortical tissue samples. Protein expression was compared between ACC ( n = 42), adrenal cortical adenoma (ACA; n = 50), and normal adrenal cortical tissue samples ( n = 45). Cytoplasmic expression of HK1 and PKM2 was significantly higher in ACC than in ACA ( p