Evaluation of the Biocompatibility of Water-Soluble Pristine С60 Fullerenes in Rabbit

C 60 fullerenes have proved their therapeutic effects and efficacy by the results of countless experiments. For further usage of these nanoparticles, the systematic toxicological investigations are required. Blood compatibility should be studied for C 60 fullerenes due to the potential blood contact. Currently, available data is not systematic and has not provided insights into possible side effects of C 60 fullerenes on blood components. In this study, water-soluble pristine C 60 fullerenes were tested in vitro to assess their biocompatibility in rabbit. The blood compatibility has been evaluated looking at the impact of C 60 fullerenes on erythrocyte integrity, platelet aggregation, and some blood factors involved in coagulation. Our results revealed that C 60 fullerenes cannot elicit hemolysis at studied concentrations and did not show any effect on coagulation process. C 60 fullerenes in concentration-dependent manner increased ADP-dependent platelet aggregation and changed the key kinetic parameters of these processes. C 60 fullerenes inhibited thrombin amidolytic activity but did not affect the activities of other studied coagulation factors. The prothrombotic property of C 60 fullerenes could be the potential risk factor that leads to enhancement of vascular thrombosis. The ability of fullerene to inhibit thrombin activity is important for the pharmacological use of these carbon nanoparticles as anticoagulant agents.