Development of an electroanalytical methodology using differential pulse voltammetry for amiloride determination

In this work, an analytical electrochemical method was developed using a glassy carbon electrode (GCE) for the determination of amiloride (AMI). To study the electrochemical behavior of AMI, the cyclic voltammetry and differential pulse voltammetry techniques were used, adding a AMI solution in 0.1 mol L −1 BR buffer solution (pH 2.0) to the electrochemical cell. A reversible AMI oxi-reduction process was revealed in the scan rate of + 0.4 to − 0.1 V. The DPV parameters optimized were the scan rate (10 mV s −1 ) and amplitude (70 mV). After parameters optimization, the linear working range was from 9.90 to 90.9 μmol L −1 , with detection limit of 5.19 μmol L −1 and quantification limit of 17.3 μmol L −1 . The validity of the electroanalytical method for the determination of AMI in biological fluids (urine and serum) was demonstrated. The proposed method is advantageous due to its good sensitivity, speed, simplicity, precision, and accuracy towards AMI quantification.