Enzyme Sampling: Temporal Sampling of Enzymes from Live Cells by Localized Electroporation and Quantification of Activity by SAMDI Mass Spectrometry (Small 26/2020)

Enzyme Sampling: Temporal Sampling of Enzymes from Live Cells by Localized Electroporation and Quantification of Activity by SAMDI Mass Spectrometry (Small 26/2020)

In article number 2000584, Milan Mrksich, Horacio D. Espinosa, and co‐workers report on a method for non‐destructively sampling enzymes multiple times from small populations of cells, using localized electroporation in microfluidic live cell analysis devices. The enzymes are captured in extraction c...

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Veröffentlicht in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2020-07, Vol.16 (26), p.n/a
Hauptverfasser: Mukherjee, Prithvijit, Berns, Eric J., Patino, Cesar A., Hakim Moully, Elamar, Chang, Lingqian, Nathamgari, S. Shiva P., Kessler, John A., Mrksich, Milan, Espinosa, Horacio D.
Format: Artikel
Sprache:eng
Schlagworte:
cell sampling
Electroporation
enzyme activities
Enzymes
Ions
Mass spectrometry
Microfluidics
Monolayers
nanotechnologies
Nanotechnology
Sampling
Scientific imaging
Spectroscopy
Substrates
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Zusammenfassung:In article number 2000584, Milan Mrksich, Horacio D. Espinosa, and co‐workers report on a method for non‐destructively sampling enzymes multiple times from small populations of cells, using localized electroporation in microfluidic live cell analysis devices. The enzymes are captured in extraction chambers, where they modify immobilized substrates on a self‐assembled monolayer, and their activity is quantified using self‐assembled monolayers for matrix‐assisted laser desorption/ionization (SAMDI) mass spectrometry.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.202070144