A Study of the Macrophage Differentiation of Acute Myeloid Leukemia Cells in Multicellular Aggregates

—The resistance of leukemic cells to the action of chemotherapeutic drugs is the main reason for insufficient efficacy in the treatment of acute myeloid leukemia. Microenvironmental factors play one of the key roles in the formation of the phenotype of drug resistance of leukemic cells. The elucidation of the nature of drug resistance mediated by the conditions of the microenvironment is important for the identification of new pharmacological targets for conservative directed therapy. We conducted a study of the role of differentiation in the macrophage direction in increasing the drug resistance of acute myeloid leukemia cells in multicellular aggregates. It was shown that maturation does not occur in the macrophage direction in multicellular aggregates. These data indicate that other mechanisms contribute to an increased drug resistance of acute myeloid leukemia cells in multicellular aggregates.