Identification and characterization of Methyl succinate from Bacillus velezensis AR1

Identification of antifungal organic compound from B. velezensis AR1 culture supernatant was performed using high-performance liquid chromatography (HPLC) and subjected to proton ( 1 H) and carbon ( 13 C) nuclear magnetic resonance (NMR). From the analysis of the heteronuclear multiple bond correlation, the purified compound was identified as monomethyl succinate (MMSucc). The subsequent investigation of its antifungal activity demonstrated growth inhibition of 5.73 to 100%, 8.75 to 96.67%, and 7.17 to 71.3% on Rhizoctonia solani, Sclerotinia sclerotiorum and Botrytis cinerea at 25 and 550 µg mL − 1 MMSucc, respectively. The minimum inhibitory concentration was 550, 650 and 675 µg mL − 1 for R. solani, S. sclerotiorum and B. cinerea , respectively. Therefore, based on its broad antifungal activities, it is possible to suggest the compound as an alternative source of fungicide.