Reaction Kinetic Analysis of Manganese Peroxidase Augmented Aerobic Waste Degradation
Abstract Past research has shown that waste degradation in landfills could be affected by augmenting with manganese peroxidase (MnP) enzyme via increased hydrolysis of organic waste. However, at present there are no quantification methods available to quantify the effects. Since kinetic parameters a...
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| Veröffentlicht in: | Journal of hazardous, toxic and radioactive waste toxic and radioactive waste, 2020-10, Vol.24 (4) |
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| creator | Bartholameuz, Eranda M Hettiaratchi, J. Patrick A Steele, Matthew Kumar, Sunil |
| description | Abstract
Past research has shown that waste degradation in landfills could be affected by augmenting with manganese peroxidase (MnP) enzyme via increased hydrolysis of organic waste. However, at present there are no quantification methods available to quantify the effects. Since kinetic parameters are direct indicators of waste degradation performance, they can be used to provide an estimate of enzyme-augmented waste degradation. A new reaction kinetic model was developed for enzyme-catalyzed reactions to determine the relevant reaction kinetic parameters. Three important kinetic parameters (overall reaction kinetic constant, hydrolysis rate constant, and oxygen assimilation constant) were quantified and compared for uncatalyzed and enzyme-catalyzed reactions. The results showed that enzyme augmentation does not yield an increase in overall waste degradation proportionate to hydrolysis of waste. Nevertheless, the overall waste degradation rate was three times higher for enzyme-catalyzed reactions than uncatalyzed reactions. This observation supports the use of MnP augmentation to increase waste degradation and as a waste stabilization strategy. |
| doi_str_mv | 10.1061/(ASCE)HZ.2153-5515.0000536 |
| format | Article |
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Past research has shown that waste degradation in landfills could be affected by augmenting with manganese peroxidase (MnP) enzyme via increased hydrolysis of organic waste. However, at present there are no quantification methods available to quantify the effects. Since kinetic parameters are direct indicators of waste degradation performance, they can be used to provide an estimate of enzyme-augmented waste degradation. A new reaction kinetic model was developed for enzyme-catalyzed reactions to determine the relevant reaction kinetic parameters. Three important kinetic parameters (overall reaction kinetic constant, hydrolysis rate constant, and oxygen assimilation constant) were quantified and compared for uncatalyzed and enzyme-catalyzed reactions. The results showed that enzyme augmentation does not yield an increase in overall waste degradation proportionate to hydrolysis of waste. Nevertheless, the overall waste degradation rate was three times higher for enzyme-catalyzed reactions than uncatalyzed reactions. This observation supports the use of MnP augmentation to increase waste degradation and as a waste stabilization strategy.</description><identifier>ISSN: 2153-5493</identifier><identifier>EISSN: 2153-5515</identifier><identifier>DOI: 10.1061/(ASCE)HZ.2153-5515.0000536</identifier><language>eng</language><publisher>Reston: American Society of Civil Engineers</publisher><subject>Augmentation ; Chemical reactions ; Enzymes ; Hazardous materials ; Hydrolysis ; Landfills ; Manganese ; Manganese peroxidase ; Organic wastes ; Parameters ; Performance degradation ; Peroxidase ; Technical Papers ; Waste disposal sites</subject><ispartof>Journal of hazardous, toxic and radioactive waste, 2020-10, Vol.24 (4)</ispartof><rights>2020 American Society of Civil Engineers</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a374t-c080cfc2f033379be4faea74c15bf8f0f36cb087d3a4c890e4fddbccfcdf4a003</citedby><cites>FETCH-LOGICAL-a374t-c080cfc2f033379be4faea74c15bf8f0f36cb087d3a4c890e4fddbccfcdf4a003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttp://ascelibrary.org/doi/pdf/10.1061/(ASCE)HZ.2153-5515.0000536$$EPDF$$P50$$Gasce$$H</linktopdf><linktohtml>$$Uhttp://ascelibrary.org/doi/abs/10.1061/(ASCE)HZ.2153-5515.0000536$$EHTML$$P50$$Gasce$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,75936,75944</link.rule.ids></links><search><creatorcontrib>Bartholameuz, Eranda M</creatorcontrib><creatorcontrib>Hettiaratchi, J. Patrick A</creatorcontrib><creatorcontrib>Steele, Matthew</creatorcontrib><creatorcontrib>Kumar, Sunil</creatorcontrib><title>Reaction Kinetic Analysis of Manganese Peroxidase Augmented Aerobic Waste Degradation</title><title>Journal of hazardous, toxic and radioactive waste</title><description>Abstract
Past research has shown that waste degradation in landfills could be affected by augmenting with manganese peroxidase (MnP) enzyme via increased hydrolysis of organic waste. However, at present there are no quantification methods available to quantify the effects. Since kinetic parameters are direct indicators of waste degradation performance, they can be used to provide an estimate of enzyme-augmented waste degradation. A new reaction kinetic model was developed for enzyme-catalyzed reactions to determine the relevant reaction kinetic parameters. Three important kinetic parameters (overall reaction kinetic constant, hydrolysis rate constant, and oxygen assimilation constant) were quantified and compared for uncatalyzed and enzyme-catalyzed reactions. The results showed that enzyme augmentation does not yield an increase in overall waste degradation proportionate to hydrolysis of waste. Nevertheless, the overall waste degradation rate was three times higher for enzyme-catalyzed reactions than uncatalyzed reactions. This observation supports the use of MnP augmentation to increase waste degradation and as a waste stabilization strategy.</description><subject>Augmentation</subject><subject>Chemical reactions</subject><subject>Enzymes</subject><subject>Hazardous materials</subject><subject>Hydrolysis</subject><subject>Landfills</subject><subject>Manganese</subject><subject>Manganese peroxidase</subject><subject>Organic wastes</subject><subject>Parameters</subject><subject>Performance degradation</subject><subject>Peroxidase</subject><subject>Technical Papers</subject><subject>Waste disposal sites</subject><issn>2153-5493</issn><issn>2153-5515</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp1kE9LAzEQxYMoWGq_w6IXPWxNNsn-8bbUakVFUYvQS5jNTsqWdrcmW7Df3ixt9eRcZph5vwfzCDlndMhozK4v8_fR-GoyG0ZM8lBKJofUl-TxEen97o4Ps8j4KRk4t-hEPMtkmvTI9A1Bt1VTB49VjW2lg7yG5dZVLmhM8Az1HGp0GLyibb6rEvyYb-YrrFssg9wvC498gmsxuMW5hRI6szNyYmDpcLDvfTK9G3-MJuHTy_3DKH8KgSeiDTVNqTY6MpRznmQFCgMIidBMFiY11PBYFzRNSg5Cpxn197IstEdKI8A_0ScXO9-1bb426Fq1aDbWP-BUJJhkaSqiTnWzU2nbOGfRqLWtVmC3ilHVJalUl6SazFSXlOpSU_skPRzvYHAa_-wP5P_gD2-TeNc</recordid><startdate>20201001</startdate><enddate>20201001</enddate><creator>Bartholameuz, Eranda M</creator><creator>Hettiaratchi, J. Patrick A</creator><creator>Steele, Matthew</creator><creator>Kumar, Sunil</creator><general>American Society of Civil Engineers</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope></search><sort><creationdate>20201001</creationdate><title>Reaction Kinetic Analysis of Manganese Peroxidase Augmented Aerobic Waste Degradation</title><author>Bartholameuz, Eranda M ; Hettiaratchi, J. Patrick A ; Steele, Matthew ; Kumar, Sunil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a374t-c080cfc2f033379be4faea74c15bf8f0f36cb087d3a4c890e4fddbccfcdf4a003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Augmentation</topic><topic>Chemical reactions</topic><topic>Enzymes</topic><topic>Hazardous materials</topic><topic>Hydrolysis</topic><topic>Landfills</topic><topic>Manganese</topic><topic>Manganese peroxidase</topic><topic>Organic wastes</topic><topic>Parameters</topic><topic>Performance degradation</topic><topic>Peroxidase</topic><topic>Technical Papers</topic><topic>Waste disposal sites</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bartholameuz, Eranda M</creatorcontrib><creatorcontrib>Hettiaratchi, J. 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Patrick A</au><au>Steele, Matthew</au><au>Kumar, Sunil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reaction Kinetic Analysis of Manganese Peroxidase Augmented Aerobic Waste Degradation</atitle><jtitle>Journal of hazardous, toxic and radioactive waste</jtitle><date>2020-10-01</date><risdate>2020</risdate><volume>24</volume><issue>4</issue><issn>2153-5493</issn><eissn>2153-5515</eissn><abstract>Abstract
Past research has shown that waste degradation in landfills could be affected by augmenting with manganese peroxidase (MnP) enzyme via increased hydrolysis of organic waste. However, at present there are no quantification methods available to quantify the effects. Since kinetic parameters are direct indicators of waste degradation performance, they can be used to provide an estimate of enzyme-augmented waste degradation. A new reaction kinetic model was developed for enzyme-catalyzed reactions to determine the relevant reaction kinetic parameters. Three important kinetic parameters (overall reaction kinetic constant, hydrolysis rate constant, and oxygen assimilation constant) were quantified and compared for uncatalyzed and enzyme-catalyzed reactions. The results showed that enzyme augmentation does not yield an increase in overall waste degradation proportionate to hydrolysis of waste. Nevertheless, the overall waste degradation rate was three times higher for enzyme-catalyzed reactions than uncatalyzed reactions. This observation supports the use of MnP augmentation to increase waste degradation and as a waste stabilization strategy.</abstract><cop>Reston</cop><pub>American Society of Civil Engineers</pub><doi>10.1061/(ASCE)HZ.2153-5515.0000536</doi></addata></record> |
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| source | American Society of Civil Engineers:NESLI2:Journals:2014 |
| subjects | Augmentation Chemical reactions Enzymes Hazardous materials Hydrolysis Landfills Manganese Manganese peroxidase Organic wastes Parameters Performance degradation Peroxidase Technical Papers Waste disposal sites |
| title | Reaction Kinetic Analysis of Manganese Peroxidase Augmented Aerobic Waste Degradation |
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