Protocol of proceedings with Fusobacterium nucleatum and optimization of ABTS method for detection of reactive oxygen species

Characterization of the ability of DSM 15643 and DSM 20482 strains in the presence of Cu and H O to reactive oxygen species generation. Spectrophotometric ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) method was used. Determination of: MIC for Cu , H O and ABTS; survivability of under atmospheric oxygen exposure; the level and rate constants of free radicals production by the bacteria. in the presence of Cu and H O is able to generate free radicals. Reactive oxygen species are produced mainly outside the bacterial cell, which suggests that outer membrane proteins may be involved in oxidative process.