Influence of different extraction temperatures and methanol solvent percentages on the total phenols and total flavonoids from the heartwood and bark of Acacia auriculiformis

Acacia auriculiformis heartwood and bark were obtained, dried under shelter for 2 weeks and pulverized into powdered form to be extracted with the following extraction temperatures of 35, 55 and 75 °C and methanol solvent percentages of 55, 65 and 75 % for 3 h in a water bath. The material ratio used was 1:20 (pulverized samples: solvent). The total phenolics and flavonoids yield was determined by using a Thermo Scientific Genesys 10 UV–Visible Spectrophotometer. The optimum total phenolics and flavonoids yield were achieved by extraction with an extraction temperature of 75 °C and a methanol solvent percentage of 75 % for both the heartwood [75.44 % (total phenolics) and 36.64 % (total flavonoids)] and bark [87.18 % (total phenolics) and 99.10 % (total flavonoids)] of A. auriculiformis trees.