Evaluation on the detection of Enterocytozoon hepatopenaei in pooled DNA samples of Litopenaeus vannamei based on TaqMan qPCR
Two populations of Litopenaeus vannamei from Haiyang and Weifang in Shandong Province were sampled. TaqMan qPCR was used to measure Enterocytozoon hepatopenaei (EHP) in the shrimp hepatopancreas one by one, and then the extracted DNA samples were pooled by 5-pool (5:1), 25-pool (25:1), 50-pool (50:1...
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| Veröffentlicht in: | Yuye kexue jinzhan 2016-01, Vol.40 (3), p.122-132 |
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| Format: | Artikel |
| Sprache: | chi |
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| Zusammenfassung: | Two populations of Litopenaeus vannamei from Haiyang and Weifang in Shandong Province were sampled. TaqMan qPCR was used to measure Enterocytozoon hepatopenaei (EHP) in the shrimp hepatopancreas one by one, and then the extracted DNA samples were pooled by 5-pool (5:1), 25-pool (25:1), 50-pool (50:1), 100-pool (100:1), and 150-pool (150:1) to test the EHP in the pooled samples. The amplification used a special threshold cycle value (Cta |
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| ISSN: | 2095-9869 |