DhLHY, a Circadian Expressed Gene of Doritaenopsis Hybrid, Promotes Floral Transition in Low Temperature, but Postpones Flowering in Overexpressed Transgenic Arabidopsis

LATE ELONGATED HYPOCOTYL ( LHY ) gene is well known to be the core circadian function in Arabidopsis. In this study, DhLHY was obtained from Doritaenopsis ‘Tinny Tender’ ( Doritaenopsis Happy smile × Happy valentine), and the sequence contained 2594 base pair (bp) in whole with an open reading frame...

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Veröffentlicht in:Tropical plant biology 2020-06, Vol.13 (2), p.162-171
Hauptverfasser: Zhang, Chi, Cheng, Qian, Li, Haiyan, Luo, Ping, Cui, Yongyi
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Sprache:eng
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Zusammenfassung:LATE ELONGATED HYPOCOTYL ( LHY ) gene is well known to be the core circadian function in Arabidopsis. In this study, DhLHY was obtained from Doritaenopsis ‘Tinny Tender’ ( Doritaenopsis Happy smile × Happy valentine), and the sequence contained 2594 base pair (bp) in whole with an open reading frame (ORF) of 1962 bp encoding 653 amino acids. The putative protein of DhLHY included a HTH myb domain and a DNA binding site shared with the reported LHY family. Aligment of amino acid (aa) showed that DhLHY shared high similarity of LHY homologs from other species. Real-time quantitative reversed transcript polymerase chain reaction (qRT-PCR) analysis indicated that DhLHY was a morning-expressed circadian gene, and expressed greater in floral induction under low temperature. DhLHY was expressed both in vegetative and reproductive organs, and the highest level of its transcripts were in the pedicle and stem. Further functional study of DhLHY -overexpressed in Arobidopsis thaliana indicated the delayed bolting time and increased leaf length in transgenic plants. Results suggested that DhLHY participated in the circadian regulation and was response to the low temperature of floral induction in Doritaenopsis .
ISSN:1935-9756
1935-9764
DOI:10.1007/s12042-019-09250-2