Lactococcus protaetiae sp. nov. and Xylanimonas protaetiae sp. nov., isolated from gut of larvae of Protaetia brevitarsis seulensis

Two bacterial strains, designated 2DFWM-2 T and FW10M-9 T , were isolated from gut of larva of Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea. 16S rRNA and rpoB gene sequences showed that strain 2DFWM-2 T formed a separate branch with Lactococcus allomyrinae 1JSPR-7 T in the genus Lactococcus , adjacent to a group of Lactococcus lactis subspecies. ANI and dDDH values between 2DFWM-2 T and Lactococcus allomyrinae 1JSPR-7 T were 93.30% and 53.20%, respectively. Based on the 16S rRNA gene sequence analysis, strain FW10M-9 T was classified into the genus Xylanimonas revealing 96.9–98.5% sequence similarities with the Xylanimonas species. The ANI values of strain FW10M-9 T with the closely species Xylanimonas pachnodae NBRC 107786 T , Xylanimonas allomyrinae 2JSPR-7 T , Isoptericola variabilis JCM 11754 T and Xylanimonas cellulosilytica DSM 15894 T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively. On the basis of the phenotypic and genotypic data, it is proposed that strain 2DFWM-2 T represents a novel species of the genus Lactococcus , for which the name Lactococcus protaetiae sp. nov. is proposed, and the type strain is 2DFWM-2 T (= KACC 19320 T = NBRC 113069 T ). And, strain FW10M-9 T represents a novel species of the genus Xylanimonas , for which the name Xylanimonas protaetiae sp. nov. is proposed, and the type strain is FW10M-9 T (= KACC 19331 T = NBRC 113053 T ).