Ten novel microsatellite markers for the western mosquitofish Gambusia affinis

We isolated and characterized 10 microsatellite loci from the western mosquitofish, Gambusia affinis . Loci were screened in 30 individuals from a single location from their native range in coastal Texas. The number of alleles per locus ranged from 4 to 25, observed heterozygosity ranged from 0.40 to 0.93, and the probability of identity values from 6.0 × 10 −3 to 2.7 × 10 −1 , with an overall PI for all loci of 1.3 × 10 −13 . These new loci provide tools for examining the genetic diversity, structure, and pedigree G. affinis populations.