Isolation and characterization of novel microsatellite loci in Vaccinium arctostaphylos L

For the first time, this study has described the characterization of 25 novel polymorphic microsatellite loci from a repeat-enriched genomic library of V. arctostaphylos using a modified FIASCO method. Polymorphism of each locus was assessed in 64 individuals of the Vaccinium . The average allele number of the microsatellites, observed (H O ) and expected (H E ) heterozygosities were 5.88, 0.85 and 0.67 per locus, respectively. The polymorphic information content (PIC) value and inbreeding coefficient (F) ranged from 0.26 to 0.87 and −0.72 to 0.03, respectively. Ten of the twenty-five loci showed significant departures from Hardy–Weinberg equilibrium. These microsatellite loci could be useful to study genetic diversity and population structure of V.arctostaphylos.