Development of 15 polymorphic microsatellite markers for the shortfin mako, Isurus oxyrinchus, and cross-species amplification in lamniforme sharks

The shortfin mako ( Isurus oxyrinchus , Lamniformes) is one of common sharks caught incidentally by tuna longline and drift net fisheries in the tropical and temperate waters. However, their genetic diversity and population structure for effective management remain to be elucidated with sufficient number of genetic markers. A total of fifteen novel microsatellite markers for the shortfin mako were developed using a next generation sequencing approach. All the microsatellite loci isolated were polymorphic with 2–23 alleles, with the observed and expected heterozygosities of 0.06–1.00 and 0.06–0.93, respectively. Cross-species amplification in six other lamniforme sharks was successful at thirteen out of fifteen loci isolated. The developed microsatellite markers will be useful for the population genetic analysis of lamniforme sharks including the shortfin mako.