Thirteen novel polymorphic microsatellite markers for endangered Chinese endemic herb Dendrobium Officinale

We isolated and developed 13 novel microsatellite makers from expressed sequence tag sequences of endangered Chinese endemic herb Dendrobium officinale and examined them for 38 individuals from Jinhua, Zhejiang, Chian. These loci displayed the mean number of 4. 31 alleles per locus, and with expecte...

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Veröffentlicht in:	Conservation genetics resources 2013-06, Vol.5 (2), p.359-361
Hauptverfasser:	Lu, Jiang-Jie, Gao, Ling, Kang, Jie-Yu, Feng, Shang-Guo, He, Ren-Feng, Wang, Hui-Zhong
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Genetics and Genomics Biodiversity Biomedical and Life Sciences Conservation Biology/Ecology Ecology Evolutionary Biology Expressed sequence tags Gene mapping Genetic diversity Genetic markers Germplasm Herbs Heterozygosity Life Sciences Microsatellites Plant Genetics and Genomics Technical Note
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Zusammenfassung:	We isolated and developed 13 novel microsatellite makers from expressed sequence tag sequences of endangered Chinese endemic herb Dendrobium officinale and examined them for 38 individuals from Jinhua, Zhejiang, Chian. These loci displayed the mean number of 4. 31 alleles per locus, and with expected heterozygosity ( H e ) and observed heterozygosity ( H o ) per locus ranged from 0.4438 to 0.6842 and from 0 to 0.4211, respectively. These markers have potential for application in germplasm appraisal, genetic diversity study, genetic mapping, and molecular breeding in D. officinale and other congeneric species.
ISSN:	1877-7252 1877-7260
DOI:	10.1007/s12686-012-9803-6