Isolation and characterization of 18 microsatellites for Lycengraulis grossidens (Pisces: Clupeiformes)

Twelve polymorphic and six non-polymorphic microsatellite loci were isolated and characterized from a CT–GT enriched genomic library of Lycengraulis grossidens . The number of alleles ranged from 1 to 29. The observed and expected heterozygosity ranged from 0.19 to 0.8 and from 0.18 to 0.95, respectively. Cross-species amplification of the markers developed for L. grossidens was successful in the wild Engraulididae species Anchoa spinifer , Anchoa marinii, Anchoviella lepidentostole , Cetengraulis edentulous and Engraulis anchoita . The developed microsatellite loci are a potential powerful tool for studies of population genetics, genetic diversity and gene flow for these important food sources.