An environmental DNA assay for detecting Arctic grayling in the upper Missouri River basin, North America

An environmental DNA assay for detecting Arctic grayling in the upper Missouri River basin, North America

The upper Missouri River basin in the northwestern US contains disjunct Arctic grayling ( Thymallus arcticus ) populations of conservation concern. To assist efforts aimed at understanding Artic grayling distribution, we developed a quantitative PCR assay to detect the presence of Arctic grayling DN...

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Veröffentlicht in:Conservation genetics resources 2016-09, Vol.8 (3), p.197-199
Hauptverfasser: Carim, K. J., Dysthe, J. C. S., Young, M. K., McKelvey, K. S., Schwartz, M. K.
Format: Artikel
Sprache:eng
Schlagworte:
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Deoxyribonucleic acid
DNA
Ecology
Environmental DNA
Evolutionary Biology
Life Sciences
Nostalgia
Plant Genetics and Genomics
River basins
Rivers
Sympatric populations
Technical Note
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Zusammenfassung:The upper Missouri River basin in the northwestern US contains disjunct Arctic grayling ( Thymallus arcticus ) populations of conservation concern. To assist efforts aimed at understanding Artic grayling distribution, we developed a quantitative PCR assay to detect the presence of Arctic grayling DNA in environmental samples. The assay amplified low concentrations of Arctic grayling DNA consistently, and did not amplify non-target species, including sympatric salmonid fishes.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-016-0531-1