The complete mitogenome of Pelochelys cantorii (Guangning) and the comparative analysis of different habitats
Pelochelys cantorii has become one of the most critically endangered species in the world. The complete mitochondrial DNA (mtDNA) genome of P. cantorii (Guangning) was generated by polymerase chain reaction (PCR) amplification, primer-walking sequencing and fragment cloning. The mitochondrial genome...
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Veröffentlicht in: | Conservation genetics resources 2018-03, Vol.10 (1), p.17-20 |
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Sprache: | eng |
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Zusammenfassung: | Pelochelys cantorii
has become one of the most critically endangered species in the world. The complete mitochondrial DNA (mtDNA) genome of
P. cantorii (Guangning)
was generated by polymerase chain reaction (PCR) amplification, primer-walking sequencing and fragment cloning. The mitochondrial genome is 17,424 base pairs (bp) and contains 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes and one control region. Comparative analyses of complete mitochondrial genome sequences from different habitats, we found that the
P. cantorii (unknown)
may be wrong identified. And the phylogenetic position of
P. cantorii
also support this result, maximum likelihood (ML) and Bayesian (BI) analyses were conducted based on 12 heavy-strand PCGs and 2 rRNAs from 18 taxa.
P. cantorii (unknown)
is not closely related to other
P. cantorii
, but formed a clade with
Apalone spinifera
and
Apalone ferox
. |
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ISSN: | 1877-7252 1877-7260 |
DOI: | 10.1007/s12686-017-0752-y |