Characterization of ten polymorphic microsatellite loci in the Andean pupfish Orestias agassizii, with cross-amplification in the sympatric O. luteus

We describe the isolation and characterization of ten polymorphic microsatellite loci in the Andean pupfish Orestias agassizii . The number of alleles per locus in 25 individuals was high, varying from 5 to 25. Observed and expected mean heterozygosities were 0.750 and 0.767, respectively. The ten loci conformed to the Hardy–Weinberg equilibrium, and we did not detect linkage disequilibrium between loci. Cross-species amplification in O. luteus was entirely successful, and polymorphism was detected in all loci. One locus (A116) showed significant departure from Hardy–Weinberg equilibrium due to homozygote excess. These microsatellites will be useful for assessing the genetic structure and historical demography of Andean pupfishes, which are subject to a global collapse of their stocks.