Development and characterization of polymorphic microsatellite markers for the crested caracara, Caracara cheriway

We isolated novel microsatellites from the crested caracara ( Caracara cheriway ) with a shotgun pyrosequencing approach. We tested 80 loci for polymorphism among 20 individuals from the threatened Florida population. Fourteen loci were polymorphic. The mean number of alleles was 2.21 (range 2–3) an...

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Veröffentlicht in:	Conservation genetics resources 2015-06, Vol.7 (2), p.557-559
Hauptverfasser:	Vaughn, Erin E., Dwyer, James F., Morrison, Joan L., Culver, Melanie
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Genetics and Genomics Biodiversity Biomedical and Life Sciences Caracara cheriway Conservation Biology/Ecology Ecology Evolutionary Biology Genetic markers Heterozygosity Life Sciences Linkage disequilibrium Microsatellite Letters Microsatellites Plant Genetics and Genomics Population genetics
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Zusammenfassung:	We isolated novel microsatellites from the crested caracara ( Caracara cheriway ) with a shotgun pyrosequencing approach. We tested 80 loci for polymorphism among 20 individuals from the threatened Florida population. Fourteen loci were polymorphic. The mean number of alleles was 2.21 (range 2–3) and the mean observed heterozygosity was 0.41 (range 0.15–0.65). None of the 14 polymorphic loci exhibited significant linkage disequilibrium nor did they deviate significantly from Hardy–Weinberg expectations. We also report 16 monomorphic loci.
ISSN:	1877-7252 1877-7260
DOI:	10.1007/s12686-015-0423-9